Our work focuses on developing and applying cutting-edge quantitative proteomics tools to dissect intricate chromatin regulatory circuits and uncover the mechanisms underlying their misregulation during aging.

We study the mechanisms driving epigenetic changes with age

We develop quantitative proteomics tools for chromatin research

RESEARCH OVERVIEW

We are interested in understanding how dynamic changes in chromatin organization over the course of life impact genome function and contribute to aging. By combining cutting-edge mass spectrometry-based proteomics with genomics and biochemical methods, we aim to provide mechanistic insights into longstanding questions:

How do chromatin protein composition and epigenetic landscape of mammalian cells change throughout life?
How do these changes affect genome stability and transcription, and what role do they play in age-related functional decline and pathologies?
What are the underlying causes of epigenetic changes with age?

Our overarching goal is to identify the factors that drive deleterious epigenetic aberrations associated with aging and to dissect pathways that can be manipulated to extend a healthy human lifespan. .LEARN.MORE.

WE ARE HIRING

We are recruiting motivated and passionate individuals at all levels, including:

- Research Specialists
- Graduate Students
(Only available to students currently enrolled at Penn)
- Postdoctoral Fellows.

Are you excited about the opportunity to learn, apply, and develop cutting-edge proteomics methods and take on ambitious projects to tackle fundamental puzzles in chromatin and aging biology? .JOIN.OUR.TEAM!.

NEWS

May 2025

Warm welcome to Dr. Dhanunjay Mukhi, our new Research Associate! Dhanunjay will investigate the role of replication-independent histone variants in regulating the genome of non-dividing cells and explore their potential contribution to epigenomic changes during mammalian aging.

APRIL 2025

Andrey has been invited to serve as Guest Editor for a JoVE special issue on “Mass Spectrometry-Based Approaches in Chromatin Biology”. We welcome submissions reporting methods for quantitative analysis of histone protein and nucleic acid modifications, chromatin-associated factors, and … .READ MORE.

February 2025

The Tvardovskiy Lab is getting ready to install the Orbitrap Astral — a state-of-the-art mass spectrometer that combines the high-resolution Orbitrap and the novel Asymmetric Track Lossless (Astral) analyzer. We're excited to explore its capabilities for quantitative analysis of nuclear proteins and histone modifications. Stay tuned!

JULY 2024

Two research highlights featuring .MARCS. - the Modification Atlas of Regulation by Chromatin States - are out in Nature Reviews Genetics (link) and Epigenomics (link). We developed MARCS as an interactive web tool to communicate our findings, offering actionable insights into chromatin regulatory circuits.

MAY 2024

Andrey is thrilled to announce that he will be joining the University of Pennsylvania as an Assistant Professor in the Department of Biochemistry and Biophysics and a core member of the Epigenetics Institute in January 2025.